Micro-computed tomography for structure-function investigation

The ICRG approach uses micro-computed tomography with novel contrast agents (31), other imaging modalities (4, 9), imaging protocols (16) and processing methods to capture detailed structural information of organs, tissues and cells, and then links this to materials analyses; via mechanical testing and assays, to provide unique insight into the structure-function relationships. For example, in joints (5, 7, 25), ears (21), and tissue-engineered constructs (15).


31. B. A. Lakin, H. Patel, C. Holland, J. D. Freedman, J. S.  Shelofsky, B. D. Snyder, M. W. Grinstaff and K. S. Stok.  Contrast-enhanced CT using a cationic contrast agent enables  non-destructive assessment of the biochemical and biomechanical  properties of mouse tibial plateau cartilage, J. Orthop Res,  34(7):1130-1138, 2016.

25. C. Barnabe, S. Finzel, K. S. Stok, P. Geusens, Invited  correspondence “High-resolution peripheral quantitative CT in  rheumatology”, Nature Rev Rheum, 11(2):123, 2015.

21. L. Nimeskern, E. M. Feldmann, S. Schwarz, W. Kuo, S. Duerr, E.  Goldberg-Bockhorn, R. Müller, N. Rotter, K. S. Stok, Magnetic Resonance  Imaging of the ear for patient-specific reconstructive surgery, PLoS  One, 9(8): e104975, 2014.

16. R. J. Choo, R. Müller and K. S. Stok. Prevention of cartilage  dehydration in imaging studies with a customised humidity chamber. Rev.  Sci. Instrum., 84(9), 093703, https://dx.doi.org/10.1063/1.4820913, 2013.

15. W. Kuo, L. Nimeskern, H. Martínez Ávila, S. Hofmann, J. Freedman,  M. W. Grinstaff, R. Müller, K. S. Stok. Developing staining protocols  for visualization of tissue-engineering scaffolds using micro computed  tomography in native wet state. Proc. Gemeinsame Jahrestagung der  Deutschen, österreichischen und Schweizerischen Gesellschaft für  Biomedizinische Technik, Graz, Austria, September 19-21, Biomed. Tech.,  58(S1):4273, 2013.

9. J. P. Moodie, K. S. Stok, R. Müller, T. L. Vincent and S. J.  Shefelbine. Multimodal imaging demonstrates concomitant changes in bone  and cartilage after destabilisation of the medial meniscus and increased  joint laxity. Osteoarthr Cartilage, 19:163-170, 2011.

7. K. S. Stok, D. Noël, F. Apparailly, D. Gould, Y. Chernajovsky, C.  Jorgensen and R. Müller. Quantitative imaging of cartilage and bone for  functional assessment of gene therapy approaches in experimental  arthritis. J. Tissue Eng. Regen. Med., 4:387-394, 2010.

5. K. S. Stok, G. Pelled, Y. Zilberman, I. Kallai, D. Gazit, and R.  Müller. Revealing the interplay of bone and cartilage in osteoarthritis  through multimodal imaging of murine joints, Bone, 45:414–422, 2009.

4. K. S. Stok, and R. Müller. Morphometric characterisation of murine  articular cartilage – Novel application of confocal laser scanning  microscopy, Microsc. Res. Techniq., 72:650–658, 2009.